Boarding the research bandwagon

The story of how I joined Johns Hopkins Institute for NanoBioTechnology (INBT) is actually one of those moments where it just hits you – Why haven’t I thought about doing this before? It started with me being back at home during the winter of my sophomore year, meeting friends of my parents and answering the most common question: Where do you study? One of the reactions that stuck with me the whole night was “Wow, how does it feel to be in the center of the most cutting-edge research?” This made me realize how I’d been oblivious to one of the things I would love to get involved in.

Better one and a half years late than never, I decided to join the research bandwagon as well. I started going through the profiles of labs on Homewood campus, looking for a topic that would make me want to be there in lab every free minute during the year. I finally found one that sparked my curiosity: the Denis Wirtz Lab. Dr. Wirtz is the Smoot Professor in the Department of Chemical and Biomolecular Engineering and also the University’s Vice Provost for Research.

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Working in the lab!

Though at the time most of the stuff I read about the Wirtz lab went over my head, I knew that cancer was something I had always wanted the world to be rid of. Seeing near and dear ones succumb to it was one of the most excruciating things which I wanted no one to experience in the future. Fascinated by the approach taken by Dr. Wirtz, I shot him off an email and to my amazement, I got an email back within the hour, “Sent to my grad students, look forward to working with you. d”. The next day I was scheduled to be back in Baltimore, and the day after that, I was a part of Wirtz lab.

During my training, I remember asking one of my peers “How in the world can I remember all these procedures, let alone do them?” She simply smiled and said, “You’ll see”. In a few weeks, I found myself doing those very procedures, one step after another as if it were a reflex action. I would most definitely attribute me being able to do this to my grad student Hasini Jayatilaka (don’t kill me for calling you out!). At the end of the day, what I felt it boiled down to, was realizing that the person I work for was in the same shoes five-seven years ago as I was now, and she wouldn’t expect anything unrealistic out of me. Once you embrace the challenge ahead, knowing that there is no need to be intimidated, you’re good to go.

The best part of being involved in research, apart from the work you do, is sitting in class in a lecture hall and suddenly tune in to the professor talking about something that you do in lab each day. That moment cements your understanding of why you did what you’ve been doing for so many days, it connects the dots in your mind, and that moment is when you’ve completed the full circle between theory and practice.

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Our team at a poster presentation over the summer.

For me personally, having to come to lab got me into a disciplined schedule. I had a fixed time for all days of the week now to wake up (which for me used to be the latest possible time before), since if I had no morning classes, I was in lab. It helped me a lot with my time management skills, with me cutting down on TV shows and sporadic naps. To my surprise, it did not affect the amount of time I spent with my friends, as the reduction in TV shows and naps was (extremely) disturbingly enough to keep every other aspect of my daily schedule the same. Being surrounded in lab by people in similar academic disciplines also gets me a ton of advice on classes. It’s like my own little “rate-my-professor” that encourages me to definitely take some class if it is “the best class I will take at Hopkins”. At times, it’s also a ‘learning den’ where I can get help with classes if I need to. Getting involved in a research lab also came with social outings with the team, with our dinners enabling us to get to know each other on a more personal level. I feel that this in a big way contributed to the chemistry we have while working with each other, made us comfortable spending time with each other at work.

At this point, after nine exciting months, including a fully lab-packed summer, I feel that this continues to be one of the best decisions I made so far. I do not regret being here every day, but take pride in saying “I need to be in lab”. One of the most cherished take-away for me is developing a sense of accountability for my actions, which I feel is an important aspect in life. I would definitely encourage being involved in research while at Hopkins as you have nothing to lose but so much to gain.

Pranay Tyle, is a junior in Chemical and Biomolecular Engineering minoring in Economics, and hopes to one day manufacture low cost medicine accessible to those in dire need across the globe.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

INBT’s fall student symposium Nov. 7

An important opportunity in graduate school is to get peer and mentor feedback on results. One of the best ways to do that is to share what you have been working on with your colleagues at a symposium.

Student-organized symposia happen twice a year at INBT.

Student-organized symposia happen twice a year at INBT.

Come hear the latest updates from Johns Hopkins Institute for NanoBioTechnology’s research centers on Friday November 7 from 8 a.m. to 3 p.m. in the Great Hall at Levering on the Homewood campus! Students affiliated with laboratories from the Johns Hopkins Physical Sciences-Oncology Center, Johns Hopkins Center of Cancer Nanotechnology Excellence and INBT will present at this student-organized symposium. This event is free and open to the Johns Hopkins community. Refreshments provided

The keynote faculty speaker is Jordan Green, associate professor at Johns Hopkins Department of Biomedical Engineering. Breakfast, networking and introductions begin at 9 a.m.

Student speakers and topics include:
**Kristen Kozielski -Bioreducible nanoparticles for efficient and environmentally triggered siRNA delivery to primary human glioblastoma cells. Jordan Green Lab. 9:30-9:45 a.m.

**Angela Jimenez – Spatio-temporal characterization of tumor growth and invasion in three-dimensions (3D). Denis Wirtz Lab. 9:50-10:05 a.m.

**Charles Hu -X-ray visible stem cell delivery for cardia regenerative therapy via microfluidics-based microencapsulation. Hai-Quan Mao Lab. 10:10- 10:25 a.m.

**Max Bogorad – An engineered microvessel platform for quantitative imaging of drug permeability and absorption.  Peter Searson Lab. 10:30-10:45 a.m.

**Greg Wiedman – Peptide Mediated Methods of Nanoparticle Drug Delivery. Kalina Hristova Lab. 10:50 to 11:05 a.m.

**Jordan Green – Particle-based micro and nanotechnology to treat cancer 11:10 a.m. – 12:10 p.m.

Please RSVP on our Facebook event page here.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

Veltri presents PS-OC hosted talk on digital pathology and prostate cancer

Robert Veltri, associate professor Of Urology and Oncology at the Johns Hopkins School of Medicine and Director of the Fisher Biomarker Biorepository Laboratory, will  present the talk Quantitative Histomorphometry of Digital Pathology: Case study in prostate cancer,” to members of the Denis Wirtz Lab and the Johns Hopkins Physical Sciences-Oncology Center on Monday, December 9 at 2 p.m. in Croft G40 on the Homewood campus. Seating is limited.

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Robert Veltri

Veltri studies the biomarkers for prostate and bladder cancer and is collaborating on applications of Quantitative Digital Image Analysis (QDIA) using microscopy to quantify nuclear structure and tissue architecture. Collaborations include Case Western Reserve University biomedical engineering and the University of Pittsburgh Electrical Engineering departments studying to assess cancer aggressiveness in prostate cancer (PCa). Furthermore,  he is studying the application of molecular biomarkers for prostate (CaP) and bladder cancer (BlCa) detection and prognosis. Veltri’s work is funded by the National Cancer Institute’s PS-OC program grant), Early Detection Research Network (EDRN), and the Department of Defense related to research on Active Surveillance for PCa. He is also a co-investigator on a SBIR-I and II grant studying the application of microtransponders to multiplex molecular urine and serum biomarker testing for CaP.  Veltri has authored over 152 scientific publications and is either inventor or co-inventor on over twenty patents and two trademarks.

Veltri presents PS-OC hosted talk on digital pathology and prostate cancer

Robert Veltri, associate professor Of Urology and Oncology at the Johns Hopkins School of Medicine and Director of the Fisher Biomarker Biorepository Laboratory, will  present the talk Quantitative Histomorphometry of Digital Pathology: Case study in prostate cancer,” to members of the Denis Wirtz Lab and the Johns Hopkins Physical Sciences-Oncology Center on Monday, December 9 at 2 p.m. in Croft G40 on the Homewood campus. Seating is limited.

veltri

Robert Veltri

Veltri studies the biomarkers for prostate and bladder cancer and is collaborating on applications of Quantitative Digital Image Analysis (QDIA) using microscopy to quantify nuclear structure and tissue architecture. Collaborations include Case Western Reserve University biomedical engineering and the University of Pittsburgh Electrical Engineering departments studying to assess cancer aggressiveness in prostate cancer (PCa). Furthermore,  he is studying the application of molecular biomarkers for prostate (CaP) and bladder cancer (BlCa) detection and prognosis. Veltri’s work is funded by the National Cancer Institute’s PS-OC program grant), Early Detection Research Network (EDRN), and the Department of Defense related to research on Active Surveillance for PCa. He is also a co-investigator on a SBIR-I and II grant studying the application of microtransponders to multiplex molecular urine and serum biomarker testing for CaP.  Veltri has authored over 152 scientific publications and is either inventor or co-inventor on over twenty patents and two trademarks.

Game Theory and Cancer

What does game theory and cancer have to do with each other. I am not sure but this interesting workshop hosted by the Princeton Physical Sciences-Oncology Center and Johns Hopkins University might help you figure that out.

An announcement about the event reads:

Screen Shot 2013-08-02 at 12.03.06 PMRegistration is now open for the Workshop on Game Theory and Cancer, scheduled on August 12-13 in Baltimore, MD, and jointly hosted by our Princeton PS-OC and Johns Hopkins University. The main goal of this workshop is to provide a dialogue between leading basic researchers and clinical investigators that would help make headway against the very stubborn problem of cancer, and to jolt the oncology community into confronting the serious clinical problems that have previously been avoided.

The flyer is pretty cool, too.  Check it out here.

Additional information and preliminary agenda can be found at: http://www.princeton.edu/psoc/training/

To register, please go to: https://prism.princeton.edu/ps-oc/regform.php

For questions about the event, email maranzam@princeton.edu or sclam@princeton.edu

Lab coats are summer gear for high school researchers

You don’t think of a lab coat as summer wear for teens, but we don’t quite feel like it’s summer around here until our research interns have arrived. Early in June, INBT’s undergraduate nano-bio researchers arrived. This week our high schoolers in the Summer Academic Research Experience (SARE) scholars got started.

SARE pairs specially selected teens with university mentors who guide them through a mini research project. At the end of their time here, they hold a small poster session. The students gain valuable work skills, learn about scientific careers, get tutoring help, practice their writing, gather data for their projects and earn some cash for the future. Students in the program are recruited from the Boys Hope Girls Home of Baltimore program, The SEED School of Maryland and The Crossroads School, all of which assist in differing ways with in the education, housing, tutoring  and counseling of promising young people from disadvantaged circumstances.

The SARE program was launched in 2009 by Doug Robinson, professor in the cell biology department at the School of Medicine, and is funded jointly by the medical school and Johns Hopkins Institute for NanoBioTechnology.

This year’s SARE scholars include: Diana Bobb is being mentored by Makoto Tanigawa in the Takanari Inoue Lab in the Department of Cell Biology; Kaleel Byrd is being mentored by Ryan Vierling in the Caren Meyers Lab in the Department of Pharmacology; Milan Dower is being mentored by Tom Lampert in the Peter Devreotes Lab in the Department of Cell Biology; Jewel Herndon is being mentored by Herschel Wade in his lab in the Department of Biophysics; De’Sean Markley is being mentored by Hoku West-Foyle in the Douglas Robinson Lab in the Department of Cell Biology

Fraley nets $500K Burroughs Wellcome Fund award for microfluidics work

Stephanie Fraley (Photo: Homewood Photography)

Stephanie Fraley (Photo: Homewood Photography)

A Johns Hopkins research fellow who is developing novel approaches to quickly identify bacterial DNA and human microRNA has won the prestigious $500,000 Burroughs Wellcome Fund (BWF) Career Award at the Scientific Interfaces. The prize, distributed over the next five years, helps transition newly minted PhDs from postdoctoral work into their first faculty positions.

Stephanie Fraley is a postdoctoral fellow working with Samuel Yang, MD, in Emergency Medicine/Infectious Disease at the Johns Hopkins School of Medicine and Jeff Wang, PhD, in Biomedical Engineering with appointments in the Whiting School of Engineering and the medical school. The goal of her work is to develop engineering technologies that can diagnose and guide treatment of sepsis, a leading cause of death worldwide, while simultaneously leading to improved understanding of how human cells and bacterial cells interact.

“Sepsis is an out of control immune response to infection,” Fraley said. “We are developing tools that are single molecule sensitive and can rapidly sort and detect bacterial and host response markers associated with sepsis. However, our devices are universal in that they can be applied to many other diseases.”

Fraley is using lab-on-chip technology, also known as microfluidics, to overcome the challenges of identifying the specific genetic material of bacteria and immune cells. Her technology aims to sort the genetic material down to the level of individual sequences so that each can be quantified with single molecule sensitivity.

“Bacterial DNA is on everything and contamination is everywhere, so trying to find the ones associated with sepsis is like the proverbial search for the needle in the haystack,” Fraley said. “With microfluidics, we can separate out all the bacterial DNA, so instead of a needle in a haystack, we have just the needles.”

Another advantage to Fraley’s novel technology is that it will assess all the diverse bacterial DNA present in a sample, without presuming which genetic material is important. “Bacteria are constantly evolving and becoming drug resistant,” she said. “With this technology, we can see all the bacterial DNA that is present individually and not just the strains we THINK we need to look for.”

Fraley’s award will follow her wherever her career takes her. The first two years of the prize fund postdoctoral training and that last three years help launch her professional career in academia. During the application process, she had to make a short presentation on her proposal to BWF’s panel of experts. “It was like the television show ‘Shark Tank’ but for scientists,” she laughs. “ The panelists gave me many helpful suggestions on my idea.”

Fraley earned her bachelor’s degree in chemical engineering from the University of Tennessee at Chattanooga and her doctorate in chemical and biomolecular engineering with Denis Wirtz, professor and director of Johns Hopkins Physical Sciences-Oncology Center. Wirtz is associate director for the Institute for NanoBioTechnology and Yang and Wang also are INBT affiliated faculty members.

BWF’s Career Awards at the Scientific Interface provides funding to bridge advanced postdoctoral training and the first three years of faculty service. These awards are intended to foster the early career development of researchers who have transitioned or are transitioning from undergraduate and/or graduate work in the physical/mathematical/computational sciences or engineering into postdoctoral work in the biological sciences, and who are dedicated to pursuing a career in academic research. These awards are open to U.S. and Canadian citizens or permanent residents as well as to U.S. temporary residents.

Landmark physical characterization of cancer cells completed

An enormous collaborative effort between a multitude of academic and research centers has characterized numerous physical and mechanical properties on one identical human cancer cell line. Their two-year cooperative study, published online in the April 26, 2013 journal Science Reports, reveals the persistent and agile nature of human cancer cells as compared to noncancerous cells. It also represents a major shift in the way scientific research can be accomplished.

Human breast cancer cells like these were used in the study. (Image created by Shyam Khatau/ Wirtz Lab)

Human breast cancer cells like these were used in the study. (Image created by Shyam Khatau/ Wirtz Lab)

The research, which was conducted by 12 federally funded Physical Sciences-Oncology Centers (PS-OC) sponsored by the National Cancer Institute, is a systematic comparison of metastatic human breast-cancer cells to nonmetastatic breast cells that reveals dramatic differences between the two cell lines in their mechanics, migration, oxygen response, protein production and ability to stick to surfaces. They have also discovered new insights into how human cells make the transition from nonmalignant to metastatic, a process that is not well understood.

Denis Wirtz, a Johns Hopkins professor of chemical and biomolecular engineering with joint appointments in pathology and oncology who is the corresponding author on the study, remarked that the work adds a tremendous amount of information about the physical nature of cancer cells. “For the first time ever, scientists got together and have created THE phenotypic signature of cancer” Wirtz said. “Yes, it was just one metastatic cell line, and it will require validation with many other cell lines. But we now have an extremely rich signature containing many parameters that are distinct when looking at metastatic and nonmetastatic cells.”

Wirtz, who directs the Johns Hopkins Physical Sciences-Oncology Center, also noted the unique way in which this work was conducted: all centers used the same human cell line for their studies, which makes the quality of the results unparalleled. And, since human and not animal cells were used, the findings are immediately relevant to the development of drugs for the treatment of human disease.

“Cancer cells may nominally be derived from the same patient, but in actuality they will be quite different because cells drift genetically over just a few passages,” Wirtz said.  “This makes any measurement on them from different labs like comparing apples and oranges.” In this study, however, the genetic integrity of the cell lines were safeguarded by limiting the number times the original cell cultures could be regrown before they were discarded.

The nationwide PS-OC brings together researchers from physics, engineering, computer science, cancer biology and chemistry to solve problems in cancer, said Nastaran Zahir Kuhn, PS-OC program manager at the National Cancer Institute.

“The PS-OC program aims to bring physical sciences tools and perspectives into cancer research,” Kuhn said. “The results of this study demonstrate the utility of such an approach, particularly when studies are conducted in a standardized manner from the beginning.”

For the nationwide project, nearly 100 investigators from 20 institutions and laboratories conducted their experiments using the same two cell lines, reagents and protocols to assure that results could be compared. The experimental methods ranged from physical measurements of how the cells push on surrounding cells to measurements of gene and protein expression.

“Roughly 20 techniques were used to study the cell lines, enabling identification of a number of unique relationships between observations,” Kuhn said.

Wirtz added that it would have been logistically impossible for a single institution to employ all of these different techniques and to measure all of these different parameters on just one identical cell line. That means that this work accomplished in just two years what might have otherwise taken ten, he said.

The Johns Hopkins PS-OC made specific contributions to this work. Using particle-tracking microrheology, in which nanospheres are embedded in the cell’s cytoplasm and random cell movement is visually monitored, they measured the mechanical properties of cancerous versus noncancerous cells. They found that highly metastatic breast cancer cells were mechanically softer and more compliant than cells of less metastatic potential.

Using 3D cell culturing techniques, they analyzed the spontaneous migratory potential (that is, migration without the stimulus of any chemical signal) of cancerous versus noncancerous cells. They also analyzed the extracellular matrix molecules that were deposited by the two cell lines and found that cancerous cells deposited more hyaluronic acid (HA). The HA, in turn, affects motility, polarization and differentiation of cells.  Finally, the Hopkins team measured the level of expression of CD44, a cell surface receptor that recognizes HA, and found that metastatic cells express more CD44.

The next steps, Wirtz said, would be to validate these results using other metastatic cell lines.  To read the paper, which is published in an open access journal, follow this link: http://www.nature.com/srep/2013/130422/srep01449/full/srep01449.html

Excerpts from original press release by Princeton science writer Morgan Kelly were used.

 

 

 

 

Recent publications from the Johns Hopkins Physical Sciences-Oncology Center

Johns Hopkins Physical Sciences-Oncology Center has had a productive quarter publishing from February to May 2013. Here are some of the most recent publications in support or the center’s core research projects, including a huge collaborative work drawing on the knowledge and research findings of the entire PS-OC network.

Screen Shot 2013-05-15 at 4.27.37 PMThat paper, A physical sciences network characterization of non-tumorigenic and metastatic cells, was the work of 95 authors from all 12 of the National Cancer Institute’s PS-OC  program centers. JHU’s PS-OC director Denis Wirtz, the Theophilus H. Smoot Professor in the Johns Hopkins Department of Chemical and Ciomolecular Engineering, is the corresponding author on this massive effort. We will be discussing the findings of that paper in a future post here on the PS-OC website. Until then, here is a link to that network paper and 13 other recent publications from the Johns Hopkins PS-OC.

  • A physical sciences network characterization of non-tumorigenic and metastatic cells.Physical Sciences – Oncology Centers Network, Agus DB, Alexander JF, Arap W,Ashili S, Aslan JE, Austin RH, Backman V, Bethel KJ, Bonneau R, Chen WC,Chen-Tanyolac C, Choi NC, Curley SA, Dallas M, Damania D, Davies PC, Decuzzi P,Dickinson L, Estevez-Salmeron L, Estrella V, Ferrari M, Fischbach C, Foo J,Fraley SI, Frantz C, Fuhrmann A, Gascard P, Gatenby RA, Geng Y, Gerecht S,Gillies RJ, Godin B, Grady WM, Greenfield A, Hemphill C, Hempstead BL, HielscherA, Hillis WD, Holland EC, Ibrahim-Hashim A, Jacks T, Johnson RH, Joo A, Katz JE,Kelbauskas L, Kesselman C, King MR, Konstantopoulos K, Kraning-Rush CM, Kuhn P,Kung K, Kwee B, Lakins JN, Lambert G, Liao D, Licht JD, Liphardt JT, Liu L, LloydMC, Lyubimova A, Mallick P, Marko J, McCarty OJ, Meldrum DR, Michor F,Mumenthaler SM, Nandakumar V, O’Halloran TV, Oh S, Pasqualini R, Paszek MJ,Philips KG, Poultney CS, Rana K, Reinhart-King CA, Ros R, Semenza GL, Senechal P,Shuler ML, Srinivasan S, Staunton JR, Stypula Y, Subramanian H, Tlsty TD, TormoenGW, Tseng Y, van Oudenaarden A, Verbridge SS, Wan JC, Weaver VM, Widom J, Will C, Wirtz D, Wojtkowiak J, Wu PH.  Sci Rep. 2013 Apr 25;3:1449. doi:10.1038/srep01449. PubMed PMID: 23618955; PubMed Central PMCID: PMC3636513. http://www.ncbi.nlm.nih.gov/pubmed/23618955
  • Procollagen Lysyl Hydroxylase 2 Is Essential for Hypoxia-Induced Breast Cancer Metastasis. Gilkes DM, Bajpai S, Wong CC, Chaturvedi P, Hubbi ME, Wirtz D, Semenza GL.Mol Cancer Res. 2013 May 7. [Epub ahead of print] PubMed PMID: 23378577. http://www.ncbi.nlm.nih.gov/pubmed/23378577
  • Predicting how cells spread and migrate: Focal adhesion size does matter. Kim DH, Wirtz D. Cell Adh Migr. 2013 Apr 29;7(3). [Epub ahead of print] PubMed PMID: 23628962. http://www.ncbi.nlm.nih.gov/pubmed/23628962
  • Hypoxia-inducible Factor 1 (HIF-1) Promotes Extracellular Matrix Remodeling under Hypoxic Conditions by Inducing P4HA1, P4HA2, and PLOD2 Expression in Fibroblasts. Gilkes DM, Bajpai S, Chaturvedi P, Wirtz D, Semenza GL. J Biol   Chem. 2013 Apr 12;288(15):10819-29. doi: 10.1074/jbc.M112.442939. Epub 2013 Feb 19. PubMed PMID: 23423382; PubMed Central PMCID: PMC3624462. http://www.ncbi.nlm.nih.gov/pubmed/23423382
  • Perivascular cells in blood vessel regeneration. Wanjare M, Kusuma S, Gerecht S. Biotechnol J. 2013 Apr;8(4):434-47. doi: 10.1002/biot.201200199. PubMed PMID: 23554249. http://www.ncbi.nlm.nih.gov/pubmed/23554249
  • Focal adhesion size uniquely predicts cell migration. Kim DH, Wirtz D. FASEB J. 2013 Apr;27(4):1351-61. doi: 10.1096/fj.12-220160. Epub 2012 Dec 19. PubMed PMID: 23254340; PubMed Central PMCID: PMC3606534. http://www.ncbi.nlm.nih.gov/pubmed/23254340
  • Notch4-dependent Antagonism of Canonical TGFβ1  Signaling Defines Unique Temporal Fluctuations of SMAD3 Activity in Sheared Proximal Tubular Epithelial Cells. Grabias BM, Konstantopoulos K. Am J Physiol Renal Physiol. 2013 Apr 10. [Epub ahead of print] PubMed PMID: 23576639. http://www.ncbi.nlm.nih.gov/pubmed/23576639
  • Integration and regression of implanted engineered human vascular networks during deep wound healing. Hanjaya-Putra D, Shen YI, Wilson A, Fox-Talbot K, Khetan S, Burdick JA, Steenbergen C, Gerecht S. Stem Cells Transl Med. 2013 Apr;2(4):297-306. doi: 10.5966/sctm.2012-0111. Epub 2013 Mar 13. PubMed PMID: 23486832. http://www.ncbi.nlm.nih.gov/pubmed/23486832
  • Collagen Prolyl Hydroxylases are Essential for Breast Cancer Metastasis. Gilkes DM, Chaturvedi P, Bajpai S, Wong CC, Wei H, Pitcairn S, Hubbi ME, Wirtz D, Semenza GL. Cancer Res. 2013 Mar 28. [Epub ahead of print] PubMed PMID: 23539444. http://www.ncbi.nlm.nih.gov/pubmed/23539444
  • Simultaneously defining cell phenotypes, cell cycle, and chromatin modifications at single-cell resolution.Chambliss AB, Wu PH, Chen WC, Sun SX, Wirtz D.FASEB J. 2013 Mar 28. [Epub ahead of print] PubMed PMID: 23538711.http://www.ncbi.nlm.nih.gov/pubmed/23538711
  • Interstitial friction greatly impacts membrane mechanics. Wirtz D. Biophys J.2013 Mar 19;104(6):1217-8. doi: 10.1016/j.bpj.2013.02.003. Epub 2013 Mar 19.PubMed PMID: 23528079; PubMed Central PMCID: PMC3602747.http://www.ncbi.nlm.nih.gov/pubmed/23528079
  • Functional interplay between the cell cycle and cell phenotypes. Chen WC, Wu PH, Phillip JM, Khatau SB, Choi JM, Dallas MR, Konstantopoulos K,Sun SX, Lee JS, Hodzic D, Wirtz D.Integr Biol (Camb). 2013 Mar;5(3):523-34. doi:10.1039/c2ib20246h. PubMed PMID: 23319145 http://www.ncbi.nlm.nih.gov/pubmed/23319145
  • High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity. Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J, Vortmeyer AO, Miller-Jensen K, Wirtz D, Fan R. Anal Chem. 2013 Feb 19;85(4):2548-56. doi:10.1021/ac400082e. Epub 2013 Feb 1. PubMed PMID: 23339603; PubMed Central PMCID:  PMC3589817.http://www.ncbi.nlm.nih.gov/pubmed/23339603

 

Microscopic grippers used successfully in animal biopsies

Tiny, untethered microscale grippers have been successfully used to perform tissue biopsies in live animals, a study in the journal Gastroenterology reports. Researchers affiliated with the Johns Hopkins School of Medicine, Whiting School of Engineering and Institute for NanoBiotechnology developed the self-assembling microgrippers, called mu-grippers. The star-shaped devices use the animal’s own body heat to trigger them to clamp down around tissue to grab a sample like a tiny hand. Because the grippers are magnetic, they can later be retrieved for a minimally invasive procedure.

Dozens of dust-sized surgical mu- grippers in a vial. (Photo by  Evin Gultepe, Gracias Lab, Johns Hopkins University)

Dozens of dust-sized surgical mu- grippers in a vial. (Photo by Evin Gultepe, Gracias Lab, Johns Hopkins University)

David Gracias, the principal investigator for the study and associate professor of chemical and biomolecular engineering, was quoted in a Johns Hopkins press release about the work: “This is the first time that anyone has used a sub-millimeter-sized device — the size of a dust particle — to conduct a biopsy in a live animal … That’s a significant accomplishment. And because we can send the grippers in through natural orifices, it is an important advance in minimally invasive treatment and a step toward the ultimate goal of making surgical procedures noninvasive.”

Read more here.